Hello, again!
I’ve been getting into the informatics side of 454 sequencing more lately, and have some questions. Mostly about barcodes…
1. Does anybody look for barcodes that shouldn’t be in a dataset? If so, how often do you find them?
2. Everything I’ve read lists an error rate of ~1% for the 454. Does this sound right?
3. Is there a normal error rate for barcodes?
Basically, I have one set of samples, using older, shorter barcodes, and keep finding other barcodes within this set. They occur at a low rate, ~.2%, but I have no way of knowing if they are real sequences or if they are occurring because of PCR errors.
Any help or advice would be greatly appreciated! Thank you!!
I’ve been getting into the informatics side of 454 sequencing more lately, and have some questions. Mostly about barcodes…
1. Does anybody look for barcodes that shouldn’t be in a dataset? If so, how often do you find them?
2. Everything I’ve read lists an error rate of ~1% for the 454. Does this sound right?
3. Is there a normal error rate for barcodes?
Basically, I have one set of samples, using older, shorter barcodes, and keep finding other barcodes within this set. They occur at a low rate, ~.2%, but I have no way of knowing if they are real sequences or if they are occurring because of PCR errors.
Any help or advice would be greatly appreciated! Thank you!!
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