Hi everyone,
We have to do a 454 GS junior run but amplimers coming up from our two steps PCR have a visible primer dimer. Do you think this will influence the quality of the run (more short reads, etc) or the AMpure purification is sufficient to get rid of all dimers?
Thank you.
We have to do a 454 GS junior run but amplimers coming up from our two steps PCR have a visible primer dimer. Do you think this will influence the quality of the run (more short reads, etc) or the AMpure purification is sufficient to get rid of all dimers?
Thank you.
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