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  • yaximik
    Senior Member
    • Apr 2011
    • 199

    bacterial genome re-sequencing

    I have a customer that wants to re-sequence 24 different bacterial genomes. What would be a reasonable level of multiplexing on GS Jr assuming an average genome size of 5-6 Mb and optimal (from your experience) for resequencing coverage depth?
  • MEJ
    Junior Member
    • Dec 2009
    • 4

    #2
    A coverage depth of 15-20x in our hands on a FLX+ is more then enough for a 2Mb bacterial genome. More coverage didn't lead to less contigs.

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