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  • Paired-end data question

    Hello all!
    I have a question about paired-end data. We have a client who is trying to gain information about a 2Kb region from a 750Kb genome and is planning to get several runs worth of FLX+ data. He already has a bit of Illumina data and a reference genome, but the reference is missing this particular region. We’re planning on performing paired-end sequencing, and our initial thoughts were to use the 3Kb protocol, but would appreciate an outside opinion. Any thoughts or recommendations?

    Thank you!

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