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  • Paper: Fully automated process for barcoded libraries for 454

    Check this out for automated 454 general library prep:



    "A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454"

  • #2
    Very cool. I wish we could just buy it off the shelf and have it working tomorrow!

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    • #3
      This is based on the old library protocol right? Now the new rapid library protocol solved a lot of issues.. but it wold be interesting to apply the 96 parallelization idea of the paper to the new rapid protocol

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      • #4
        Fluidigm have a 454 amplicon prep method that allows you to make 48 libraries of up to 480 amplicons, barcoded and ready to sequence. All from one PCR.

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        • #5
          Oh yes, this thing? http://www.fluidigm.com/pr/2010/2010...essRelease.htm

          Looks really handy for some of our apps.

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