I would also love an explanation of the 454 quality scores.
I think there is an error in the original definition of 454 quality scores, as described here: http://seqanswers.com/forums/showthread.php?p=50237
Cheers,
T
-
From the manual:
"The signal flowgrams of the reads are basecalled and quality scores for each base are calculated based on the signal properties relative to the statistical distributions of the Control DNA fragment signals."
Does that help?Leave a comment:
-
Flowgram signals to quality score
Can anyone explain how the raw flowgram signals are converted into quality scores for the 454 GSFLX system? I'm trying to compare two processing methods, and am running into trouble with each screening for quality based on different criteria (one based on flowgram signal, the other based on quality score).
Thanks!
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Leave a comment: