Tweet
Hi,
anyone used MIRA? Can't find the answer to this in the manual or online...
I need to filter some 454 paired read data before assembly, using a custom blastdb. That part I have working ok. I've used sff_extract.py to get a fastq and trace_info.xml file for input to MIRA, which I am assembling minus filtering. But, what I need to know is, if I filter the reads, thus loosing the traceinfo.xml file, will MIRA still do the proper clipping of the fastq (remove lower case sequences?)?
Thanks for any help, I have posted this to the mira list but thought I'd ask here too just in case anyone knew..
S.
anyone used MIRA? Can't find the answer to this in the manual or online...
I need to filter some 454 paired read data before assembly, using a custom blastdb. That part I have working ok. I've used sff_extract.py to get a fastq and trace_info.xml file for input to MIRA, which I am assembling minus filtering. But, what I need to know is, if I filter the reads, thus loosing the traceinfo.xml file, will MIRA still do the proper clipping of the fastq (remove lower case sequences?)?
Thanks for any help, I have posted this to the mira list but thought I'd ask here too just in case anyone knew..
S.
Comment