Dear all, I performed sequence capture of target exons using NimbleGen SeqCap EZ strategy. Now I have to proceed to emPCR (Lib L). Should I denature my for 95 C degrees for 2 minutes?
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After sequence capture I usually end up between 1 and 2 cpb; so if you measured your sample with a Qubit before calculating the nr of molecules my guess would be that 1cpb would be a safe number.
Usually I do one SVE with 1 cpb and one with 2 cpb and after calculating enrichment I choose a cpb for my bulk emulsion.
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