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No. of reads related to regions



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  • No. of reads related to regions

    I was wondering if anyone knows the number of reads related to number of regions for 454 sequencing. I know they have an equation to calculate it.

    Anyone knows the roughly number of reads I could possible get.

    1 region: XXX reads

    2 regions: XXX reads

    4 regions: XXX reads,

    8 regions: XXX reads

    16 regions: XXX reads


  • #2
    This is the number of beads you load onto the plate:

    2 region plate = 2 million per region = 4 million total
    4 region plate = 780.000 per region = 3,12 million
    8 region plate = 340.000 per region = 2,72 million total
    16 regions plate = 125.000 per region = 2 million total

    usually I end op with less than half the reads


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