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Hi, I'm writing my thesis and I'm trying to figure out what is the probability of sequencing a read once you put it into the 454. I know that you will loose some reads at emPCR step and then in the sequencing. So the question is if I feed the 454 with 1 million reads how many will probably be sequenced?
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Hi aleferna,
Copying from a paper "Using only one nanogram of fragmented DNA, we prepared ... 53.6 millions Y library molecules, which is sufficient for 10 Titanium runs (4 million enrichment beads to yield 1 million high quality reads per run) for Roche 454 Titanium sequencing".
If you have 1M library molecules, beads recovery around 70 ~ 90%, one fourth beads being sequenced, you might end up around 200,000 reads.
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