Does anyone know what's going on with all the supply interruptions at Roche/454? I can't seem to get a straight answer about anything from them.
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Originally posted by rbarry View Postunfortunately all she will tell me is that she wishes she knew more
My guess is that either this QC step is now a bottleneck or that so many lots of product from this vendor are being rejected that the supply of this vendor's product is bottlenecking the supply of emPCR kits.
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Phillip
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My guess is that either this QC step is now a bottleneck or that so many lots of product from this vendor are being rejected that the supply of this vendor's product is bottlenecking the supply of emPCR kits.
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Originally posted by rbarry View PostThey are telling me now that it's the XLR70 sequencing kits that are on backorder with no projected date as to when they will be available.
Anyone having trouble getting emPCR kits?
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Phillip
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Originally posted by pmiguel View PostAnyone having trouble getting emPCR kits?
The XLR70 kits are supposed to ship as soon as the last component holding up the QC passes.Last edited by 454User; 05-11-2010, 04:32 PM.
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[QUOTE=pmiguel;18390]Well, that is mysterious. I inferred that the problem was with the emPCR kits, because when I mentioned the "Low Number of Raw Wells" issue I was having at the User's Group meeting, the problem was immediately blamed on issues with emPCR kits.
Hi Phillip,
Could you tell more about "Low number of raw wells" issue. We have this issue almost every run lately and reported to Roche multiple times, but I hear from from you first time that this is not only our problem and the cause is emPCR kit.
Thanks,
Vlad
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[QUOTE=vlee2;18550]Originally posted by pmiguel View PostCould you tell more about "Low number of raw wells" issue. We have this issue almost every run lately and reported to Roche multiple times, but I hear from from you first time that this is not only our problem and the cause is emPCR kit.
Reminds me of the Titanium kits in the first half of last year which completely failed to give good data on GC rich (~ >= 62%) genomes or genome regions. Has never been made really public and I still meet people who paid quite some money and ask why they don't get good assemblies. The questions they ask and the symptoms they report make me immediately think that they must have sequenced it in that time frame. I'm right 90% of the time.
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[QUOTE=vlee2;18550]Originally posted by pmiguel View PostWell, that is mysterious. I inferred that the problem was with the emPCR kits, because when I mentioned the "Low Number of Raw Wells" issue I was having at the User's Group meeting, the problem was immediately blamed on issues with emPCR kits.
Hi Phillip,
Could you tell more about "Low number of raw wells" issue. We have this issue almost every run lately and reported to Roche multiple times, but I hear from from you first time that this is not only our problem and the cause is emPCR kit.
Thanks,
Vlad
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Originally posted by 454User View PostI just placed an order today for several SVemPCR kits and was informed that they are on backorder indefinitely.
The XLR70 kits are supposed to ship as soon as the last component holding up the QC passes.
Big shipment is said to be going out today.
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[QUOTE=gilly;18556]Originally posted by vlee2 View Post
Hi we've seen this too. 750K for each region on a 2 region plate when we'd expect 1.1 million. We’ve re-counted the beads and I’m sure we’re loading the correct amount.
I have delved into this issue previously:
(Where I come to the wrong conclusion about the cause of the issue. We corrected our deviation from protocol, and the results did not change.)
and a related thread about what does constitute a raw well:
Basically the results we had been getting around that time, despite the lower numbers of raw wells, were decent, so we were not employing coercive interrogation methods on Roche representatives. Now the consensus seems to be: problem with multiple emPCR kits/batches.
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Phillip
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