Hi guys,
For my reasearch I am studying both DNA and RNA viruses. Currently I extract both partitions from a sample and treat them as separate samples. For my RNA I perform a whole transcriptome amplification, effectively converting all the RNA into cDNA.
My question is - once I have converted my RNA to cDNA is it possible for me to mix both the cDNA and DNA fractions in order to prepare and run them at once on the GS Juinor platform? A Roche representative advised me not to do this but did not really clarify why. I cannot seem to think of any reason why it wouldn't work or how it would interfere with a successful run.
Has anyone here tried doing this?
Thank you.
For my reasearch I am studying both DNA and RNA viruses. Currently I extract both partitions from a sample and treat them as separate samples. For my RNA I perform a whole transcriptome amplification, effectively converting all the RNA into cDNA.
My question is - once I have converted my RNA to cDNA is it possible for me to mix both the cDNA and DNA fractions in order to prepare and run them at once on the GS Juinor platform? A Roche representative advised me not to do this but did not really clarify why. I cannot seem to think of any reason why it wouldn't work or how it would interfere with a successful run.
Has anyone here tried doing this?
Thank you.
Comment