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  • styagi
    started a topic 454 high-throughput Amplicon sequencing project

    454 high-throughput Amplicon sequencing project

    Hi Everyone,
    I have recently acquired 454 amplicon sequences. It is vital that I use a alignment method that does not depend on breaking the reads into smaller ones like BWA. I want to use a program that will align long reads to the reference and also perform variant calling. I am in the process of using 454 gsAmplicon for the alignment and wanted to ask if there are other good softwares for 454 amplicon long read alignment.

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  • seqadmin
    Recent Developments in Metagenomics
    by seqadmin





    Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
    09-23-2024, 06:35 AM
  • seqadmin
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    Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
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