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  • 454 high-throughput Amplicon sequencing project

    Hi Everyone,
    I have recently acquired 454 amplicon sequences. It is vital that I use a alignment method that does not depend on breaking the reads into smaller ones like BWA. I want to use a program that will align long reads to the reference and also perform variant calling. I am in the process of using 454 gsAmplicon for the alignment and wanted to ask if there are other good softwares for 454 amplicon long read alignment.

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  • seqadmin
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    11-06-2024, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR
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    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM

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