Does anyone know how to edit reads after alignment has been done using gsAmplicon software? I want to go back and edit any variants that I know are sequencing errors.
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I just found that there is no function to edit reads on the 454 software. Does anyone know a program that would allow me to edit reads post alignment. I dont want filter out the reads completely, I want to be able to edit the errors and use them for alignment and analysis.
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From what I understand they'll give you the programs you need if you ask nicely:
http://seqanswers.com/forums/showpos...8&postcount=13
I think there also are some open source variants of the tools but I never used them.
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Originally posted by Zaag View PostWell if that really is what you want you could go from sff to fasta, alter the fasta and go back to sff. You can use the sfftools/sffinfo provided by Roche.
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