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  • Tech Summary: Roche's 454 GS20 / FLX / Titanium

    There isn't anything here yet!

    If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.

    Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".

  • #2
    quick summary of titanium from 454 site

    Needed this for something else may be incomplete or wrong but ..

    400-600 million high-quality, filter-passed bases per run
    10 hours
    average length 400 bp
    more than 1x10^6 reads

    a cluster from roche is recommended for signal processing from www.pssclabs.com. It looks like image processing will still happen on the GS-FLX and 9G of cwf (wells) files need to go the the cluster

    from what I can tell it is a 5 node system (20 cores) the only specs I can find say redhat with either openMPI or MPI2 (mpiexec), SunGrid as scheduler

    runs will be 35GB (26GB images)

    A test data-set with a configuration tester is promised soon

    Rumor has it that bead breaking will be via centrifuge in the new protocol

    Comment


    • #3
      I am confused by this .. "pyrosequencing from illumina" !
      --
      bioinfosm

      Comment


      • #4
        Originally posted by bioinfosm View Post
        I am confused by this .. "pyrosequencing from illumina" !
        Confused here too.
        Illmunia currently produces 15-20GB from single run of paired-end already.
        Protocol will be available sometime early next year.

        Comment


        • #5
          Originally posted by bioinfosm View Post
          I am confused by this .. "pyrosequencing from illumina" !
          Originally posted by monad View Post
          Confused here too.
          Illmunia currently produces 15-20GB from single run of paired-end already.
          Protocol will be available sometime early next year.
          They bought Avantome.

          Comment


          • #6
            SV

            Look out for the SV prep on titanium. There is a pretty serious typo under the DNA capture step. 3.2.7 the volume to evenly distribute capture beads is off. It says 40ul per rxn, it's really more like 70+ul. In fact completely ignore this section and prep each rxn in individual 1.7ml tubes. The roche tech agrees with me... just wash with 3-400ul of cap bead wash buff so you don't run out.... Then again, does Ti even work as advertised?

            Comment


            • #7
              Hi,

              You have already summary out the tech of Roche's 454 GS20 / FLX / Titanium?
              I interesting about it too

              Originally posted by ECO View Post
              There isn't anything here yet!

              If you are interested in writing a tech summary, just post it below and I will rearrange the thread as appropriate.

              Alternatively if anyone wants to post tech sheets, manuals, etc, I will write it. I just don't know enough of it beyond "pyrosequencing, picotiter plates, and a ton of beads".

              Comment


              • #8
                Cangs

                Does anybody already have to use CANGS? If yes he could explain to me why it filters primers sequences while this filter is already made by 454?

                Comment


                • #9
                  With Illumina now going to 300bp, does there remain a viable role for 454?

                  Comment


                  • #10
                    Originally posted by scrosby View Post
                    With Illumina now going to 300bp
                    link?

                    --
                    Phillip

                    Comment


                    • #11
                      Forgive me...

                      I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?

                      Comment


                      • #12
                        Actually, with the results I saw from recent v3 runs I was wondering if anyone had pushed out past 100 nt on HiSeq chemistry already?

                        --
                        Phillip

                        Comment

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