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Does anyone have 454 Junior XL+ seq reagents/enzymes currently in your freezer? I need 1 kit but Roche hasn't been able to provide it for the past 2 months.
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Sorry, but we haven't been able to get reagents for months also. We have some students who need to graduate and expected these data for their dissertations. Everyone's mad at the Core of course. We have a backlog of about 12 runs... maybe 20. If anyone has a stash, after mps4208, please consider helping us out also.
On the other hand, at least those few still working on kits are paying attention to quality control... at least that's the hope.
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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