Header Leaderboard Ad

Collapse

Any aftermarket 454 reagents being (or going to be) produced?

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Any aftermarket 454 reagents being (or going to be) produced?

    So when the Roche 454 reagents dry up, will no other company step up with compatible kits? I notice Epicentre has dropped all their Nextera stuff.

    Will Roche make the reagents public in case we want to try and make our own kits? We can pretty much guess at a lot of the protocols, but it would be great to just have a cookbook method available.

    I asked about "repurposing" the 454-Junior we have, but no ideas so far.

  • #2
    I haven't heard anything yet, but we are hoping the same thing.

    Epicentre is slowly shifting everything to Illumina, at least Illumina picked up the Nextera.

    Comment


    • #3
      Reagents are one thing, but I can't imagine anyone would want to lay out for manufacturing picotiter plates.

      Comment


      • #4
        We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them. Also, the channel glass they are made from has been around for a couple decades now (before 454 technology) and will still be made.

        Comment


        • #5
          Originally posted by hoytpr View Post
          We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them.
          And that is exactly what ruined a number of projects. I spent insane amount of time cleaning up contaminated projects. Don't do it. Although some labs could be more thorough in sonication and water exchange, clearly the beads not only do remain in the PTP plate but even the enzymes keep working in a following sequencing. Feel free to place an order on my website www.bioinformatics.cz for cleanup of your current data. ;-)

          Comment

          Latest Articles

          Collapse

          • seqadmin
            A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
            by seqadmin


            ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

            01-24-2023, 01:19 PM
          • seqadmin
            Introduction to Single-Cell Sequencing
            by seqadmin
            Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

            The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
            ...
            01-09-2023, 03:10 PM

          ad_right_rmr

          Collapse
          Working...
          X