Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Overcalled A's in my 454 data

    I've assembled 780,000 reads of 454 sequence with the Roche gsAssembler program and I've noticed that there are a huge number of overcalled A's. Most of them seem to be associated with runs of A's, which is no surprise, but the fact that its all one base, and almost always an insertion. Its almost like the settings for acquiring that channel on the instrument were off. It looks like the program has collapsed the gaps caused by these insertions in the final contigs, but I'm wondering if this is inhibiting some of the contigs from being linked.

    Is there anyway to "turn down the A's", or "re-call" the bases in the data?

    Thanks

Latest Articles

Collapse

  • seqadmin
    Advanced Methods for the Detection of Infectious Disease
    by seqadmin




    The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases.
    ...
    11-27-2023, 01:15 PM
  • seqadmin
    Strategies for Investigating the Microbiome
    by seqadmin




    Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...
    11-09-2023, 07:02 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 10:35 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-05-2023, 02:24 PM
0 responses
17 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-05-2023, 07:37 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-04-2023, 08:23 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Working...
X