Dear all,
I am new to NGS and need some advise please.
Would it be sensible to use deep sequencing of PCR amplicons (e.g. 16S gene in genomic DNA) for quantitative studies of complex microbial population in a number of samples? Will this sequence informantion retain the initial proportional composition of the populations and can it be used for a comparative quantification of population composition between the samples? If so, how many PCR cycles should be used for the amplification to avoid bias?
I hope it all makes sense..
Thanks
I am new to NGS and need some advise please.
Would it be sensible to use deep sequencing of PCR amplicons (e.g. 16S gene in genomic DNA) for quantitative studies of complex microbial population in a number of samples? Will this sequence informantion retain the initial proportional composition of the populations and can it be used for a comparative quantification of population composition between the samples? If so, how many PCR cycles should be used for the amplification to avoid bias?
I hope it all makes sense..
Thanks
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