Hi,
we are currently trying to decide how to process our 16S amplicons on titanium. How would I come to a rough estimate of expected reads per sample on 1/8th PTP at 16-plex? (assuming everything is perfectly normalized).
I am under the impression that splitting the plate reduces sequencing output overall but am unsure as to how great a decrease to expect in output to expect.
Cheers,
Paul
we are currently trying to decide how to process our 16S amplicons on titanium. How would I come to a rough estimate of expected reads per sample on 1/8th PTP at 16-plex? (assuming everything is perfectly normalized).
I am under the impression that splitting the plate reduces sequencing output overall but am unsure as to how great a decrease to expect in output to expect.
Cheers,
Paul
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