Hello Everyone,
I have recently used a custom Agilent SureSelect bait library to enrich several Illumina multiplexed libraries (combined and loaded on a single lane). I have left over bait library and would like to use it to do the same thing with Rapid 454 MID libraries. I recently found out that additional Rapid MID adaptors can now be purchased from IDT. These MID adaptors are delivered as two oligos that must be annealed into a double-stranded adaptor prior to library preparation.
I wanted to know.....
1)Can the separate oligos (not annealed) be used for Linker Mediated PCR (LM-PCR) to amplify sample libraries pre and post hybridization with the SureSelect baits?
2)Can the separate oligos (not annealed) be used as blocking oligos in the hybridization?
Thanks for your help!
DoubleA
I have recently used a custom Agilent SureSelect bait library to enrich several Illumina multiplexed libraries (combined and loaded on a single lane). I have left over bait library and would like to use it to do the same thing with Rapid 454 MID libraries. I recently found out that additional Rapid MID adaptors can now be purchased from IDT. These MID adaptors are delivered as two oligos that must be annealed into a double-stranded adaptor prior to library preparation.
I wanted to know.....
1)Can the separate oligos (not annealed) be used for Linker Mediated PCR (LM-PCR) to amplify sample libraries pre and post hybridization with the SureSelect baits?
2)Can the separate oligos (not annealed) be used as blocking oligos in the hybridization?
Thanks for your help!
DoubleA
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