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  • frossit
    replied
    Thank you very much

    Leave a comment:


  • rosatoc
    replied
    check ABRF

    The DNA Sequencing Research Group (DSRG) of ABRF presented this topic at the last meeting. A link to their page is here:


    Under the Electronic Poster section, #1, there is a downloadable .pdf on the topic. And the membership of the RG can be asked about further information (e.g. #2 in on-going Studies).

    I hope this helps,
    Caprice

    Leave a comment:


  • frossit
    replied
    Hi
    I´m new here
    Do you have any presentation in ppt or pdf about Targeting sequencing or enrichment genomic regions
    Thanks

    Leave a comment:


  • tanmoy_img
    replied
    thank you very much for the help.
    regards,
    Tanmoy

    Leave a comment:


  • sigusn
    replied
    We don't have anything published on Mouse yet. But here are some papers:
    This one tests Nimblegen capture
    Although oligonucleotide arrays are quick and easy to develop, some problematic regions may evade capture, necessitating sequential redesigning for complete optimization. Neither sequencing technology was able to detect every variant identified by Sanger sequencing because of well-known drawbacks of …

    Using Nimblegen capture on Horses
    We report a high-quality draft sequence of the genome of the horse (Equus caballus). The genome is relatively repetitive but has little segmental duplication. Chromosomes appear to have undergone few historical rearrangements: 53% of equine chromosomes show conserved synteny to a single human chromo …

    On cattle
    PMID: 20865119
    Nimblgen has a collection of papers, user guides and protocols

    I hope that helps something
    regards,
    Snaevar

    Leave a comment:


  • tanmoy_img
    replied
    Hi sigusn....
    Thank you for your reply....can u suggest me some research papers.
    rgds,
    T

    Leave a comment:


  • sigusn
    replied
    Hi
    We have done Nimblegen sequence capture on dogs, horses, cows, mouse, humans, etc. All works fine. Just using the standard protocol from Nimblegen+Illumina should work fine, or use your own library preparation variants.

    Leave a comment:


  • tanmoy_img
    replied
    An urgent help needed regarding sequence caputure

    Hi.....
    I want some information regarding nimbulgen sequence capture of mouse genome.I want to sequence4.5 Mbp region on x chromosome of few mouse samples.can anybody share there experience with mouse samples.All kind of recommendations are welcome.Please send me some references or protocols or articles on the same.Please help.
    Best regards,
    Tanmoy

    Leave a comment:


  • giakr84
    replied
    Hi all, do you know an alternative method to perform a microarray experiment using 454 Junior? (no Nimblegen)
    Thanks

    Leave a comment:


  • vasvale
    replied
    imp[rovements for the library prep in this paper:
    Nat Methods. 2008 Dec;5(12):1005-10.

    A large genome center's improvements to the Illumina sequencing system.

    Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ, Durbin R, Swerdlow H,
    Turner DJ.

    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
    Cambridgeshire, CB10 1SA, UK.

    Leave a comment:


  • sci_guy
    replied
    Nimblegen are hosting a webinar very soon. See here.

    Leave a comment:


  • ATGC_man
    replied
    Hello,

    HD2 is available from Nimblegen !

    Anyone have more information about that ?

    Leave a comment:


  • Chipper
    replied
    Originally posted by poliveira View Post
    Hi all!

    My first time here so be gentle
    My question is: have you any idea whether the sequencing results will allow the user to distinguish strand orientation... This because I am interested in transcriptome analysis and the strand info is crutial... Let me know please, I'm drowning in papers...
    Cheers to all and thanks to the threading you all are introducing me to a brand new world!
    ciao!
    You will only be able to tell the direction / strand of the sequence you read, not the transcript. If you have polymorphisms in your exons you might see some strand specificity though. But for transcriptiome sequencing you do not need the enrichment arrays I guess.

    Leave a comment:


  • bioinfosm
    replied
    Not sure if I understood you right, but Yes. The alignment algorithm will tell you if the sequence aligned to forward/reverse strand of the reference sequence you provide..

    Leave a comment:


  • poliveira
    replied
    Hi all!

    My first time here so be gentle
    My question is: have you any idea whether the sequencing results will allow the user to distinguish strand orientation... This because I am interested in transcriptome analysis and the strand info is crutial... Let me know please, I'm drowning in papers...
    Cheers to all and thanks to the threading you all are introducing me to a brand new world!
    ciao!

    Leave a comment:

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