[frossit]

Thank you very much

[rosatoc]

check ABRF

The DNA Sequencing Research Group (DSRG) of ABRF presented this topic at the last meeting. A link to their page is here:

http://www.abrf.org/index.cfm/group.show/DNASequencing.28.htm

Under the Electronic Poster section, #1, there is a downloadable .pdf on the topic. And the membership of the RG can be asked about further information (e.g. #2 in on-going Studies).

I hope this helps,
Caprice

[frossit]

Hi
I´m new here
Do you have any presentation in ppt or pdf about Targeting sequencing or enrichment genomic regions
Thanks

[tanmoy_img]

thank you very much for the help.
regards,
Tanmoy

[sigusn]

We don't have anything published on Mouse yet. But here are some papers:
This one tests Nimblegen capture

http://www.ncbi.nlm.nih.gov/pubmed/20562348

Using Nimblegen capture on Horses

http://www.ncbi.nlm.nih.gov/pubmed/19892987

On cattle
PMID: 20865119
Nimblgen has a collection of papers, user guides and protocols

http://www.nimblegen.com/products/lit/index.html

I hope that helps something
regards,
Snaevar

[tanmoy_img]

Hi sigusn....
Thank you for your reply....can u suggest me some research papers.
rgds,
T

[sigusn]

Hi
We have done Nimblegen sequence capture on dogs, horses, cows, mouse, humans, etc. All works fine. Just using the standard protocol from Nimblegen+Illumina should work fine, or use your own library preparation variants.

[tanmoy_img]

An urgent help needed regarding sequence caputure

Hi.....
I want some information regarding nimbulgen sequence capture of mouse genome.I want to sequence4.5 Mbp region on x chromosome of few mouse samples.can anybody share there experience with mouse samples.All kind of recommendations are welcome.Please send me some references or protocols or articles on the same.Please help.
Best regards,
Tanmoy

[giakr84]

Hi all, do you know an alternative method to perform a microarray experiment using 454 Junior? (no Nimblegen)
Thanks

[vasvale]

imp[rovements for the library prep in this paper:
Nat Methods. 2008 Dec;5(12):1005-10.

A large genome center's improvements to the Illumina sequencing system.

Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ, Durbin R, Swerdlow H,
Turner DJ.

Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
Cambridgeshire, CB10 1SA, UK.

[sci_guy]

Nimblegen are hosting a webinar very soon. See here.

[ATGC_man]

Hello,

HD2 is available from Nimblegen !

Anyone have more information about that ?

[Chipper]

Hi all!

My first time here so be gentle
My question is: have you any idea whether the sequencing results will allow the user to distinguish strand orientation... This because I am interested in transcriptome analysis and the strand info is crutial... Let me know please, I'm drowning in papers...
Cheers to all and thanks to the threading you all are introducing me to a brand new world!
ciao!

You will only be able to tell the direction / strand of the sequence you read, not the transcript. If you have polymorphisms in your exons you might see some strand specificity though. But for transcriptiome sequencing you do not need the enrichment arrays I guess.

[bioinfosm]

Not sure if I understood you right, but Yes. The alignment algorithm will tell you if the sequence aligned to forward/reverse strand of the reference sequence you provide..

[poliveira]

Hi all!

My first time here so be gentle
My question is: have you any idea whether the sequencing results will allow the user to distinguish strand orientation... This because I am interested in transcriptome analysis and the strand info is crutial... Let me know please, I'm drowning in papers...
Cheers to all and thanks to the threading you all are introducing me to a brand new world!
ciao!