After a short spell of good results, again we're getting way short reads on our 454 Titanium WGS runs. Last time this was down to dodgy consumables and Roche put a release out admitting there was a QC problem. Anyone else having problems recently?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
The engineer came:
It seems some of the tubes inside were dirty and wash flow may have been insufficient which means removal of residual dNTP after each cycle would be incomplete and so as the reads progress, there is increasing corruption of the read sequence due to overlapping reads after displacement of the end of the strand 'forwards' by illegitimate insertion of bases that should not be available in the next cycle (if I've understood correctly), a phenomenon called read-forward. Apparently Roche's analysis of the data suggested that this was indeed the problem.
This suggests to us we need to make sure the tubes are replaced at no more than 12 mo intervals and possibly as little as 6 mo.
(Edited this post for clarity)Last edited by nickloman; 11-17-2010, 06:39 AM.
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
23 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment