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  • Sequencing reagents lot 93801720

    I was wondering if anyone had experienced problems with the above sequencing reagents lot?

    We performed a run a couple of days ago which has not generated a single read. The Raw wells for this 4 region run was around 1.3 million but both the sample key pass and control key pass were both 0. It maybe a problem with the instrument or technician but as this was the first run we’ve performed using this lot I thought I’d ask if anyone else was having problems with it.

  • #2
    If there are 0 wells for control beads, then there is likely one of 2 problems:
    1. the control beads weren't added (likely)
    2. there is a problem with the instrument (only if you didn't get anything from the sample beads too

    I would start by rerunning and making sure the control beads were added in the correct volume. If you still don't get any results for the control beads, then 454 needs to come look at your instrument.

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    • #3
      Was the sequence primer annealed? (Edit: you would of course still have control beads with a sequence primer so stupid question on a mondaymorning)

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      • #4
        We used this lot successfully on 4 runs in October.

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