Hello everybody,
we have problems with the final library size selction and the ampure beads. After the two cut-offs, we always get to small fragments. Did anybody tried to do the size selection by cutting it out of a gel instead of the beads? And maybe is it possible to do this at an earlier stage, when the carrier DNA is still in the sample, so you would actually see something on the gel?
Thanks a lot and good luck with your stuff.
Gecko
we have problems with the final library size selction and the ampure beads. After the two cut-offs, we always get to small fragments. Did anybody tried to do the size selection by cutting it out of a gel instead of the beads? And maybe is it possible to do this at an earlier stage, when the carrier DNA is still in the sample, so you would actually see something on the gel?
Thanks a lot and good luck with your stuff.
Gecko
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