Analysis of the 454 results

[Zoe rui]

Hi,all!
I'm fresh in 454 sequencing and here, but I am keen to know more information on this field. Then I have a simple question:
>contig00005 length=47 numreads=179 gene=isogroup00001 status=it_thresh
what's the meaning of the "numreads" and "gene=isogroup00001 "?
Thanks very much.
Regards;
Zoe.

[westerman]

numreads should be, I hope, obvious. It is simply the number of reads making up the contig.

gene is the large grouping from which the contig arises. Looking at the 454 Newbler manual will give a better feel for the differences between contigs and isogroups -- you really need to know this if you are doing 454 sequencing. Also I and others have written about isogroups in SeqAnswers. Take a search through the forum.

[pmiguel]

I recommend reading Lex's (flxlex) blog:

http://contig.wordpress.com/

In depth information on GS-FLX software.

--
Phillip

[Zoe rui]

Thanks so much