As of this week we have completed over 1,500 ChIP-Seq samples on the Helicos. A few nanograms, no library creation, just a little poly A tailing and zoom, 9-14 million reads on 46 samples every 14 days (we like to relax before we fill it up again.).
Just don't ask me why they just got delisted on NASDAQ and are trading at 22 cents. I have no idea. ;-( Keith Robison spells it out pretty well in the other thread. Their marketing should have been something like "It's all about the message" and focused on ChIP-Seq, RNA-Seq, DGE and all of the above from paraffin, nanogram amounts, no amplification, no bias.
They should have never chased after the human genome. Wrong tool for the job.
-Paul Morrison
-
Dana Farber has a Helicos. Contact Paul Morrison there.
Leave a comment:
-
Does anyone have any experience with Helicos sequencing
Hello Everyone,
I was wondering if anyone has any experience in Helicos sequencing. I saw the technology on its website, and it looked pretty robust. I was wondering what were its pros and cons compared to SoliD and Illumina and it was something one would want to invest in.
Thanks
Abhijit
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Leave a comment: