Sample purification question

glassfish

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Join Date: Jun 2011

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#1

Sample purification question

06-30-2011, 07:55 AM

Hi all,

I am going to drop off my first batch of DNA samples for sequencing next week. I plan to do a paired-end 101 sequencing of GAII. I sheared the DNA to 200bp by a Covarit. I plan to purify the DNA fragments with QIAquick gel extraction kit, but the technician from our core facility told me I don't need to purify them. I just want to check whether it is normal... Thanks a lot!
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jjenrette

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Join Date: Nov 2010

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#2

07-05-2011, 11:22 AM

It is normal. You do not need to purify. The library prep protocol will contain many purification steps along the way.
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protist

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Join Date: Jan 2009

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#3

07-06-2011, 01:10 AM

There is no need to gel purify - we do run the fragmented material through a Qiagen PCR column but that is only to concentrate the sample.
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