Have you ever read such report? seems I found some sequence error hotspots.
-
-
Error "hotspots" exist for every technology I've looked at.
E.g.:- older Sanger ABIs (370 and 373) had the "AG-problem".
- 454 has the homopolymer problem. To be honest, Sanger sequencing and Solexa also have it ... but much less pronounced.
- for the Solexa projects I've analysed, I've seen strong bias after GGC.G, see also http://seqanswers.com/forums/showthread.php?t=1353
SOLiD I have no idea, I never analysed that data.
Regards,
B. -
Hey Bach, Thanks for your response, we plan to go deep into the sequence error, hope can get more general ideas.
now we get too much AC misidentification, and seems they cluster into several positions.Last edited by mingkunli; 03-19-2009, 07:49 AM.Comment
-
I guess that is due to A and C intensities being so similar.. and phasing makes it worse with increasing cycle-number--
bioinfosmComment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...Yesterday, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment