I just started using CASAVA and currently demultiplexing now. Can anyone explain what exactly is demultiplexing? Having a trouble understanding the purpose of it. Thanks in advance.
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I assume you are using CASAVA version 1.8.Originally posted by cjose View Post
If you do not have "multiplexed" samples then this version of casava treats samples being multiplex of "1". When you are "de-multiplexing", the process is converting the "bcl" files (primary sequencing output) to actual FASTQ sequence files. If you do have real multiplexed samples then it will also split the reads into bins based on the "tag" info you provide in the SampleSheet file. -
It's 1.8. Thanks for the explanation.Comment
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I would also suggest using more informative subject for your question
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Haha - can't argue with you there!Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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