I constructed 3 libraries using the ScriptSeq mRNA protocol. 2 were from mRNA and 1 from small RNA. Though the sizes of the final libraries, based on the Agilent HS chip was averaging 300-400bps;the profiles had numerous peaks.There was not a uniform peak as is usually seen on completed illumina libraries. Any ideas?
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ScriptSeq mRNA library
Attaching library profile for 2 libraries labeled JJ-L8 and JJ DPP-9Comment
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Sorry for the delayed response. Those peaks are definitely not typical of the libraries that we've prepared, or data that other customers have submitted. Can you let me know the source of the RNA, how it was purified, and how much mRNA was used as input for the ScriptSeq procedure?Comment
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Hi Lisamae,Originally posted by Lisamae19 View Post
I'm wandering how was your small RNA library prepared with ScriptSeq mRNA. Did you obtain good results?
ThanksComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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