ScriptSeq mRNA library

[Lisamae19]

I constructed 3 libraries using the ScriptSeq mRNA protocol. 2 were from mRNA and 1 from small RNA. Though the sizes of the final libraries, based on the Agilent HS chip was averaging 300-400bps;the profiles had numerous peaks.There was not a uniform peak as is usually seen on completed illumina libraries. Any ideas?

[epibio]

Can you post the Bioanalyzer profiles here, or e-mail them to me? Thanks.

[Lisamae19]

ScriptSeq mRNA library

Attaching library profile for 2 libraries labeled JJ-L8 and JJ DPP-9

[epibio]

Sorry for the delayed response. Those peaks are definitely not typical of the libraries that we've prepared, or data that other customers have submitted. Can you let me know the source of the RNA, how it was purified, and how much mRNA was used as input for the ScriptSeq procedure?

[cascoamarillo]

I constructed 3 libraries using the ScriptSeq mRNA protocol. 2 were from mRNA and 1 from small RNA. Though the sizes of the final libraries, based on the Agilent HS chip was averaging 300-400bps;the profiles had numerous peaks.There was not a uniform peak as is usually seen on completed illumina libraries. Any ideas?

Hi Lisamae,

I'm wandering how was your small RNA library prepared with ScriptSeq mRNA. Did you obtain good results?

Thanks