identification of indels/duplications up to 20 bp
Hi,
Is it possible to identify insertions, deletion or duplications of up to 20 bp with target enrichment and Illumina sequencing?
What is the minimal read length required? I understand that it should be PE.
While aligning the reads to the human genome, should I change the default numbers? (I'm used to use BWA, but will it be suitable?)
I am experienced with identification of very short indels (1-2 bp) in results of illumina sequencing, but now want to know if I can use it to sequence genes were up to 20 bp indels are expected.
Thanks!
Is it possible to identify insertions, deletion or duplications of up to 20 bp with target enrichment and Illumina sequencing?
What is the minimal read length required? I understand that it should be PE.
While aligning the reads to the human genome, should I change the default numbers? (I'm used to use BWA, but will it be suitable?)
I am experienced with identification of very short indels (1-2 bp) in results of illumina sequencing, but now want to know if I can use it to sequence genes were up to 20 bp indels are expected.
Thanks!