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How many reads will be generated for each library in HiseqSQ sequencing for miRNAs? I want to know how many samples I can pool in one lane. Thank you every!!
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I think your question is better phased as "how many reads can I get from one lane from a HiSeqSQ". I presume you are actually talking about the HiScanSQ. Then knowing how many reads you need per sample you will know how many samples you can pool. We got around 82M reads per lane in our last single-end run. Theoretical is a bit more. Sample prep will play a big role; our range was from 74M to 89M. -
Thank you for reply! So, how many reads can cover all the miRNAs for each library? I want to know the maximum number of libraries that can be pooled in a lane? How many libraries can generate 82M reads in your last single-end run. Thank you so much!!!!Originally posted by westerman View PostComment
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