We're planning to add a second index to the TruSeq v2 kit because we need more multiplexing than just 24. Are there any rules on making your own index?
I asked Illumina and they said to make sure that for each cycle A,T,C,G are all represented because the MiSeq has to "focus" or else the cycle is lost. So this means I can't make a universal tag since there will be cycles where all my base reads would only consist of a single nucleotide! Is this accurate?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM