We're planning to add a second index to the TruSeq v2 kit because we need more multiplexing than just 24. Are there any rules on making your own index?
I asked Illumina and they said to make sure that for each cycle A,T,C,G are all represented because the MiSeq has to "focus" or else the cycle is lost. So this means I can't make a universal tag since there will be cycles where all my base reads would only consist of a single nucleotide! Is this accurate?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM