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  • hechanner
    Junior Member
    • Dec 2011
    • 1

    #1

    Minimum coverage needed of de novo small RNA sequencing

    Hello,

    I am a novice member with some novice questions.
    I have data about small RNA profile of an avian species without reference genome. I get less than 1M reads per sample after filtration. My questions are: 1, how many reads per sample should be enough? 2, for analysis, should I map them to an related species' genome?
    Also, I am looking for publications about de novo in small RNA.

    Thanks a lot in advance for your help!

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