I'd like to know what types of ranges people are getting for the total quantity of their final chip-seq and/or msp1-digested rrbs libraries. For chip-seq, let us assume the starting amount of DNA is 1-10ng and 15-17 cycles of post-ligation PCR with phusion taq. For rrbs let us assume the starting amount of genomic DNA is 500ng also with the same range of PCR (using Pfu Turbo Cx taq from Agilent) post-ligation and bisulfite conversion.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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