[scooter]

The frag buffer will have a 1x concentration of ~10 mM Zn2+. This is pretty high so I think any amount of EDTA that might get co-precipitated will be very low and probably not worth worrying about.

[martona]

Hi everybody,

Is anybody familiar with the fragmentation buffer below? I need to know the proportion of the below. This is what I 've found in a paper. I am trying to get fragments of about 200 bp excluding the adapters for SOLID sequencing. mRNA was fragmented at 82 degrees for 2 minutes..
Does anybody have any ideas ?

40mM Tris-acetate
100 mM Potassium acetate
31.5 mM Magnesium Acetate
pH 8.1

Did you try this buffer? I would like to use it