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  • scooter
    Member
    • Feb 2010
    • 29

    #16
    The frag buffer will have a 1x concentration of ~10 mM Zn2+. This is pretty high so I think any amount of EDTA that might get co-precipitated will be very low and probably not worth worrying about.

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    • martona
      Junior Member
      • Nov 2010
      • 3

      #17
      Originally posted by Tulay Bayram View Post
      Hi everybody,

      Is anybody familiar with the fragmentation buffer below? I need to know the proportion of the below. This is what I 've found in a paper. I am trying to get fragments of about 200 bp excluding the adapters for SOLID sequencing. mRNA was fragmented at 82 degrees for 2 minutes..
      Does anybody have any ideas ?

      40mM Tris-acetate
      100 mM Potassium acetate
      31.5 mM Magnesium Acetate
      pH 8.1
      Did you try this buffer? I would like to use it

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