Remove adaptors

[zshu]

Hi, there!

I'm pretty new in this area. I have a question. I get the Illumina RNA seq data. Do I need to trim the adaptors manually? And do all sequences share the same adaptor sequence?

Thank you!

[Wallysb01]

You should ask the sequencing core what adapter sequences were used for your libraries. Once you have those, you can remove them with a number of tools. I like scythe so far (https://github.com/vsbuffalo/scythe).

[Lanlan]

No, normally you only get sequences of your samples.

Hi, there!

I'm pretty new in this area. I have a question. I get the Illumina RNA seq data. Do I need to trim the adaptors manually? And do all sequences share the same adaptor sequence?

Thank you!