Hi,
Our lab has been optimising an Illumina PE protocol on very small amounts of DNA, ordered the PE amplification primers from the sticky on this forum so we had enough to test different PCR conditions etc.. Does anyone know whether libraries generated with these oligos will be sequencable on the GA IIx? Or do the primers in the Illumina library prep kit have modifications added on necessary for the sequencing?
Thanks,
Aaron
Our lab has been optimising an Illumina PE protocol on very small amounts of DNA, ordered the PE amplification primers from the sticky on this forum so we had enough to test different PCR conditions etc.. Does anyone know whether libraries generated with these oligos will be sequencable on the GA IIx? Or do the primers in the Illumina library prep kit have modifications added on necessary for the sequencing?
Thanks,
Aaron
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