For sequencing on illumina HiSeq, I am now making DNA libraries with different insert lengths (original lengths of just sheared DNA fragments peaked at 300, 450, and 600).
In sequencing, do you recommend not to mix two or three of them (with different insert lengths) in a single lane? Will there be any unfavorable effect caused by the different insert lengths in a lane? Maybe libraries with shorter inserts are more frequently sequenced?
In sequencing, do you recommend not to mix two or three of them (with different insert lengths) in a single lane? Will there be any unfavorable effect caused by the different insert lengths in a lane? Maybe libraries with shorter inserts are more frequently sequenced?
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