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Each MiSeq sequencing kit is for one run only and you can multiplex several libraries in one run depending on number of required reads. Multiplexing can be tailored to obtain equal or unequal number of reads for each library.
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NextSeq kit close to expire date
Does anybody know the different between NextSeq High output 300cycles and 150cycles kit? Only the reagent volume different? May I combine 2 of 150cycles kit for a 300 cycles run?Comment
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No, you couldn't. The NextSeq, like the MiSeq, has all reagents provided in a single slide-in cartridge. The instrument will read the barcode on the reagent cartridge and determine from that the number of cycles which that cartridge can be used for. There is no way to override this.Originally posted by GA-J View Post
[Disclaimer: My answer is not based on first hand knowledge but on assuming that the NextSeq operates like the MiSeq in this respect.]Comment
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Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...10-07-2024, 08:07 AM -
Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM
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