Each MiSeq sequencing kit is for one run only and you can multiplex several libraries in one run depending on number of required reads. Multiplexing can be tailored to obtain equal or unequal number of reads for each library.
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NextSeq kit close to expire date
Does anybody know the different between NextSeq High output 300cycles and 150cycles kit? Only the reagent volume different? May I combine 2 of 150cycles kit for a 300 cycles run?Comment
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No, you couldn't. The NextSeq, like the MiSeq, has all reagents provided in a single slide-in cartridge. The instrument will read the barcode on the reagent cartridge and determine from that the number of cycles which that cartridge can be used for. There is no way to override this.Originally posted by GA-J View Post
[Disclaimer: My answer is not based on first hand knowledge but on assuming that the NextSeq operates like the MiSeq in this respect.]Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
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