Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • GW_OK
    Senior Member
    • Sep 2009
    • 411

    #16
    We're very happy with Silver level. Also echoing that I wouldn't attempt to run a next-gen system of any flavor without a service contract.

    Comment

    • cdalgard
      Member
      • Apr 2011
      • 12

      #17
      illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?

      Comment

      • illuminaGA
        Member
        • Dec 2012
        • 71

        #18
        Hi,cdalgard

        I run PE(101+7+101). I stopped all the 3 test running around cycles 20. I didn't try finishing the running because the intensities form cycles 2-20 usually under 100k.

        Originally posted by cdalgard View Post
        illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?

        Comment

        • illuminaGA
          Member
          • Dec 2012
          • 71

          #19
          cdalgard- Hi, the "cycle2 issue" happened on our another GAiix. Same issue on our two marchine, that make me crazy.

          This time, the lot No. is 9303701 and 9303702

          Originally posted by cdalgard View Post
          illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?
          Last edited by illuminaGA; 08-02-2013, 12:53 PM.

          Comment

          • illuminaGA
            Member
            • Dec 2012
            • 71

            #20
            update-

            I re-Hyb the flowcell and rerun. The new running performance well. I really want to figure out what happened.

            Comment

            • cdalgard
              Member
              • Apr 2011
              • 12

              #21
              I wanted to follow up and ask if anything was resolved with your GA IIx. Is the system working for all runs or do you still have any technical problems?

              Comment

              • cdalgard
                Member
                • Apr 2011
                • 12

                #22
                Our FSE wants to know which lab you are from so they can cross reference your case report and see if a similar issue is present on our system.

                Comment

                Latest Articles

                Collapse

                • SEQadmin2
                  Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
                  by SEQadmin2



                  Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

                  Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
                  ...
                  10-14-2026, 09:55 AM
                • SEQadmin2
                  New Genomics Technologies Take Aim at Long-Standing Limits
                  by SEQadmin2


                  Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

                  We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
                  ...
                  09-28-2026, 10:25 AM

                ad_right_rmr

                Collapse

                News

                Collapse

                Topics Statistics Last Post
                Started by SEQadmin2, 10-15-2026, 10:52 AM
                0 responses
                26 views
                0 reactions
                Last Post SEQadmin2  
                Started by SEQadmin2, 10-14-2026, 05:39 AM
                0 responses
                32 views
                0 reactions
                Last Post SEQadmin2  
                Started by SEQadmin2, 10-13-2026, 09:56 AM
                0 responses
                31 views
                0 reactions
                Last Post SEQadmin2  
                Started by SEQadmin2, 10-09-2026, 11:14 AM
                0 responses
                28 views
                0 reactions
                Last Post SEQadmin2  
                Working...