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  • GW_OK
    Senior Member
    • Sep 2009
    • 411

    #16
    We're very happy with Silver level. Also echoing that I wouldn't attempt to run a next-gen system of any flavor without a service contract.

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    • cdalgard
      Member
      • Apr 2011
      • 12

      #17
      illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?

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      • illuminaGA
        Member
        • Dec 2012
        • 71

        #18
        Hi,cdalgard

        I run PE(101+7+101). I stopped all the 3 test running around cycles 20. I didn't try finishing the running because the intensities form cycles 2-20 usually under 100k.

        Originally posted by cdalgard View Post
        illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?

        Comment

        • illuminaGA
          Member
          • Dec 2012
          • 71

          #19
          cdalgard- Hi, the "cycle2 issue" happened on our another GAiix. Same issue on our two marchine, that make me crazy.

          This time, the lot No. is 9303701 and 9303702

          Originally posted by cdalgard View Post
          illuminaGA - Are these runs SR or PE? We are running PE and the strange observation is that Cycle 1 is good, Cycles 2-67 are poor, Indexing Cycles are good (and we get good multiplex efficiency), and Read 2 Cycles (another 67) are good. Illumina Tech Support is surprised that only Read 1 is affected. Do you have any similar data? Or are you only running SR?
          Last edited by illuminaGA; 08-02-2013, 12:53 PM.

          Comment

          • illuminaGA
            Member
            • Dec 2012
            • 71

            #20
            update-

            I re-Hyb the flowcell and rerun. The new running performance well. I really want to figure out what happened.

            Comment

            • cdalgard
              Member
              • Apr 2011
              • 12

              #21
              I wanted to follow up and ask if anything was resolved with your GA IIx. Is the system working for all runs or do you still have any technical problems?

              Comment

              • cdalgard
                Member
                • Apr 2011
                • 12

                #22
                Our FSE wants to know which lab you are from so they can cross reference your case report and see if a similar issue is present on our system.

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