Hi,
We are testing running Illumina DNA prep PCR free and we noticed that our colleague had used the wrong protocol for ELM, instead of 37°C for 5 minutes followed by 50°C for 5 minutes, the following protocol was used: 37°C for 5 minutes followed by 50°C for 30 sec, meaning a 4.5 min shorter run at 50°C. Does anyone know what this step does and how it can impact performance.
We are also interested in any aspects that can improve data, in particular GC rich dropout regions.
/Anna
We are testing running Illumina DNA prep PCR free and we noticed that our colleague had used the wrong protocol for ELM, instead of 37°C for 5 minutes followed by 50°C for 5 minutes, the following protocol was used: 37°C for 5 minutes followed by 50°C for 30 sec, meaning a 4.5 min shorter run at 50°C. Does anyone know what this step does and how it can impact performance.
We are also interested in any aspects that can improve data, in particular GC rich dropout regions.
/Anna
Comment