Adding adaptors on DNA samples

[Elisa]

Hello,

I have done a Crispr/cas9 screen with my durg, and now I'm preparing the DNA samples. I'm confused that if I need to add a flowcell adaptors on both ends? I have inquired a platform that they told me I only need to add illumina sequence when I amplify the samples. And also barcodes on both ends or only sing end?
For my understanding, the primer should be in this order:
A flowcell attachment sequence+barcode+illumina sequence+target binding site.
Is that right?

Thank you!

[Ben3]

Hello Elisa can you tell us a little more about which sequencer you will be running your samples on? And do you plan to do a single- or pair-end sequencing run?

[Elisa]

Hello Elisa can you tell us a little more about which sequencer you will be running your samples on? And do you plan to do a single- or pair-end sequencing run?

Hello, thank you Ben3. It seems that they will use the DNBSEQ PE100. What bother me most is that do I need two indexs or one is OK to do a paired-end sequencing?

[Ben3]

Elisa I haven't personally run a DNBSEQ sequencer before, but since it's a pair-end run, I would say that you do need two different indexes (one on each side). This is part of Complete Genomics instruments and you can learn more about that process through this link. It explains how the entire sequencing process works for your sequencer and should help answer your question.

[Elisa]

Elisa I haven't personally run a DNBSEQ sequencer before, but since it's a pair-end run, I would say that you do need two different indexes (one on each side). This is part of Complete Genomics instruments and you can learn more about that process through this link. It explains how the entire sequencing process works for your sequencer and should help answer your question.

That's very helpful for me, thank you!😀