Hello,
I have done a Crispr/cas9 screen with my durg, and now I'm preparing the DNA samples. I'm confused that if I need to add a flowcell adaptors on both ends? I have inquired a platform that they told me I only need to add illumina sequence when I amplify the samples. And also barcodes on both ends or only sing end?
For my understanding, the primer should be in this order:
A flowcell attachment sequence+barcode+illumina sequence+target binding site.
Is that right?
Thank you!
I have done a Crispr/cas9 screen with my durg, and now I'm preparing the DNA samples. I'm confused that if I need to add a flowcell adaptors on both ends? I have inquired a platform that they told me I only need to add illumina sequence when I amplify the samples. And also barcodes on both ends or only sing end?
For my understanding, the primer should be in this order:
A flowcell attachment sequence+barcode+illumina sequence+target binding site.
Is that right?
Thank you!
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