Hi
I am currently using Truseq kits. Just finished with sample prep. On gel, I could distinctively see one bright band but after gel extraction followed by PCR amplification, Bioanalyzer is showing 2 peaks - one between 200-500bp(desired) and other around 9bp-1kb!! The intensity of both are similar.
Could it be a problem with PCR kit?
I am really confused. Please suggest possible reason for this and a solution, if any.
P.S. I diluted my DNA sample with crosslinked MQ for Bioanalyzer reading?
I am currently using Truseq kits. Just finished with sample prep. On gel, I could distinctively see one bright band but after gel extraction followed by PCR amplification, Bioanalyzer is showing 2 peaks - one between 200-500bp(desired) and other around 9bp-1kb!! The intensity of both are similar.
Could it be a problem with PCR kit?
I am really confused. Please suggest possible reason for this and a solution, if any.
P.S. I diluted my DNA sample with crosslinked MQ for Bioanalyzer reading?